Acetyltransferase was detected in the E.coli recombinants, which indicates that the antibody is useful for practical studies. 制得的抗体在重组大肠杆菌里检测到了乙酰转移酶,证明所得抗体具有实验价值。
Although desirable recombinants were recovered in the progenies as expected, they generally appeared to be weak and lack of vigorous plant growth. 如所预料,重组的基因型出现于后裔,但这些品系多数显示软弱缺乏旺盛的生长力。
Recovery of Vesicular stomatitis virus Recombinants Expressing E2 Protein of CSFV 表达猪瘟病毒E2蛋白的复制型水疱性口炎假病毒的拯救
Then the recombinants were transfected into COS-7, it was proved that the env gene were expressed in the host by IFA. 中;将重组质粒转染到宿主细胞COS7细胞中,通过间接免疫荧光试验(IFA),证明该基因在宿主细胞中得到表达。
Objective: To construct the glucose-activated human proinsulin gene recombinants intensively expressing mature insulin in hepatocytes. 目的构建在肝细胞内增强表达成熟人胰岛素的生理调控性人胰岛素原基因重组质粒。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing. 通过酶切、PCR及测序鉴定各重组体。
Recombinants were determined by means of α-complementary test, agarose gel electrophoresis and dot hybridization. 经α互补检测,初步筛选重组子。随机挑取单菌落分别培养,提取质粒,经琼脂糖凝胶电泳和斑点杂交,最后确定重组子,重组率为43.8%。
The multicopy recombinants were obtained through G418 concentration gradient screening and PCR. 经G418浓度梯度筛选及PCR鉴定得到高拷贝整合的重组酵母表达菌株。
Recombinants were confirmed by colony PCR and restriction enzyme digestion. 经菌落PCR和质粒酶切鉴定后,将目的基因进行序列测定。
Objective: To identify positive clones from DNA recombinants rapidly. 目的:从DNA重组体中快速鉴定出阳性克隆。
The recombinants were obtained by the assay of PCR. 将转化子进行PCR筛选,得到酵母重组菌株。
Conclusion There are kinds of HIV-1 subtypes and recombinants among international travelers, which is the main source of imported HIV strain. 〔结论〕国际旅行人员中HIV-1亚型和重组型种类复杂多样,是我国HIV毒株输入的主要来源。
The expression of pokeweed antiviral protein gene in Pichia pastoris Mut~+ and Mut~ s recombinants 毕赤酵母Mut~+和Mut~s重组子表达美洲商陆抗病毒蛋白基因的比较
Conclusions The eukaryotic expression plasmids of Mycobacterium tuberculosis ESAT-6 antigen were successfully constructed, and the recombinants pVAC-esat-6 could induce specific humoral immunity and cellular immunity in immunized mice. 结论成功构建结核分枝杆菌抗原ESAT-6的真核表达质粒pVAC-esat-6,免疫小鼠后诱导产生了特异的体液免疫应答和细胞免疫应答。
The recombinants were identified with double GA endonuclease digestion, specific PCR and sequencing. 分别经双酶切、特异PCR及测序法对重组质粒进行鉴定。
Methods Design the ribozyme cDNA fragment and construct recombinants. 方法设计核酶cDNA序列。
Application of PCR technique in the screening of positive recombinants PCR技术在鉴定阳性重组子中的应用
Then, the sequence of the selected positive recombinants were confirmed by DNA sequencing. 经限制性酶切凝胶分析、PCR鉴定、DNA测序,筛选出阳性重组质粒。
The positive recombinants were identified by RT-PCR to amplify NEO genes. Neo基因的RTPCR法鉴定阳性克隆细胞;
Results The virus titers of both recombinants increased obviously after ping pong procedures. 结果病毒重组体的病毒滴度在乒乓超感染后有明显提高,最高病毒滴度约为1×106CFU/ml;
Percentage of recombinants was 96.2%. 重组百分比为96.2%。
Utilizing the yeast homologous recombination system, 23 recombinants were obtained; 利用酵母的同源重组系统将这26个PCR产物转入酵母,最终23个发生了重组;
A total of 44 recombinants were selected in this population. 从该F2群体中,共筛选到44个重组体。
Conclusion: The adenovirus vector of hIL-24 was successfully constructed and adenovirus recombinants had biological activity. 结论:成功构建人IL-24的重组腺病毒载体,其病毒重组子具有生物活性。
After the induction of IPTG, the recombinants were applied to conduct asymmetric reduction of 2-hydroxyacetophenone. 重组菌在IPTG诱导下进行表达,并对2-羟基苯乙酮进行不对称催化还原。