Present data indicates that, oocyte morphology, including the shape of the first polar body and the size of perivitelline space, reflects the maturity of the oocytes and may have a predictive value on the ability of fertilization following ICSI. 因此推测,卵母细胞的第一极体形态和卵周隙大小反映了卵母细胞的成熟度,受精前观察卵母细胞第一极体和卵周隙的形态可以预测ICSI后的受精能力。
The blastomeres separated from the embryos were injected into the perivitelline space of enucleated secondary oocytes under the control of micromanipulator. 在显微操作仪的控制下,将分离后的单个卵裂球注入去核次级卵母细胞的卵周隙。
After fertilization membrane was lifted, the secondary egg emitted the second polar body into the perivitelline space. 受精膜举起后,由次级卵母细胞排放出第二极体。
In order to study the influence of fertilization on the infection of embryos by lentivirus, lentivirus of low titer ( 106 TU/ ml) were microinjected into perivitelline spaces of mouse fertilized eggs and oocytes. 为了研究受精过程对慢病毒感染胚胎的影响,本试验采用卵周隙显微注射的方法,用低滴度的病毒(106TU/ml)分别感染小鼠受精卵和卵母细胞。