Polymorphisms between mtDNAs of male sterile and normal cytoplasms were detected by Southern hybridization with the probe of R 2 630 WA fragment. 以该片段为探针进行Southern杂交分析检测到在雄性不育胞质与正常可育胞质间存在的线粒体DNA多态性。
Fundamental Studies on Restriction Endonuclease Maps of mtDNAs from the Pupas of Domesticated Silkworms 家蚕蛹线粒体DNA限制酶图谱初步研究
Using PCR-SSCP, PCR-RFLP and PCR product direct sequencing techniques, we analysed the mitochondrial DNAs ( mtDNAs) of 90 patients with NIDDM ( Non Insulin-Dependent Diabetes Mellitus) and those of 80 normal controls. 采用PCR-SSCP、PCR-RFLP及PCR产物直接测序等技术对90例NIDDM(即非胰岛素依赖型糖尿病)及80例正常对照个体的血细胞线粒体DNA进行了突变分析。