Pathological significance of expression of a proliferation-inducing ligand and its receptors mRNAs in human gastric carcinoma 胃癌组织中增殖诱导配体及受体mRNAs表达的病理意义
Reverse transcriptase-polymerase chain reaction ( RT-PCR) was applied to determine the existence of sex hormone receptor mRNAs ( AR, ER α and PR) in bovine trabecular meshwork cells. Immunohistochemical technique was used to localize these receptors in bovine trabecular meshwork cell. 用逆转录聚合酶链反应的方法检测牛眼小梁组织中性激素受体蛋白mRNAs(AR,ERα和PR)的表达,用免疫组织化学技术来定位这些受体。
Localization of gaba_a and gaba_c receptor subunit mRNAs in carp retina ── an in situ hybridization analysis GABAA和GABAC受体各亚单位基因在鲫鱼视网膜内的分布&原位杂交组织化学法研究
Methods The effects of rhIL 6 on the expressions of IL 6, IL 6R, c myc, c fos and c jun mRNAs were analyzed by Northern blotting hybridization. 并应用Northern杂交检测rhIL-6对IL-6、IL-6受体(IL-6R)、c-myc、c-fos、c-jun等基因表达的影响。
Results 249 genes out of 8 192 were identified differently, at least two times. Most of the genes ( 79%) involved in cell signaling transduction, cell cycles, metabolism, inflammatory response and apoptosis, whose mRNAs were differently regulated. 结果在8192个基因中,初筛出249个(3%)表达差异达2倍以上的基因,其中主要是细胞信号和传递,细胞周期和代谢,凋亡及炎症相关类基因(占79%)。
Results: The kidney-invigorating recipe could increase the expression levels of estrogen receptor α and β mRNAs in different parts such as aorta, pituitary gland and hypothalamus, and enhance the affinity of estrogen receptor-ligands. 结果:补肾中药可提高主动脉、垂体和下丘脑雌激素受体α、β基因的表达,并提高主动脉和下丘脑组织雌激素受体-配体结合力。
Co-expression of α-cgrp,β-cgrp and PPTA mRNAs in the rat trigeminal ganglion cells α-和β-CGRP及PPTAmRNAs在大鼠三叉神经节神经元中的共同表达
In summary, mRNAs for IGF-I, IGF-I receptor, and FSH receptor were coordinately expressed in preantral and small antral follicles, which may be important in early stages of follicular development. 综上结果,提示IGF-I,IGF-I受体和FSH受体在窦前和小窦状卵泡中的协同表达对卵泡的早期发育有重要作用。
Conclusion: The therapeutic effects of SI on PMO may be related to regulating the expression levels of OPG and OPGL mRNAs, and the ratio of OPGL mRNA/ OPG mRNA. 结论:SI防治PMO的效应可能与其调控OPGmRNA的表达及OPGLmRNA/OPGmRNA比值相关。
Expression of mRNAs and protein for glial cell line-derived neurotrophic factors in bone marrow stromal cells of SD rat 大鼠骨髓基质细胞分泌胶质细胞源性神经营养因子的研究
MicroRNAs ( miRNAs) are a class of endogenous noncoding single-stranded RNA with about 22-23 nucleotides length. MicroRNA function as sequence-specific negative regulators in post-transcriptional gene silencing by base pairing with target mRNAs, which leads to mRNA cleavage or translational repression. microRNAs(miRNAs)是生物体内源长度约为20&23个核苷酸的非编码小RNA,通过与靶mRNA的互补配对而在转录后水平上对基因的表达进行负调控,导致mRNA的降解或翻译抑制。
Objective To study the expression of fibronectin and type ⅲ collagen mRNAs in rat periodontal ligament during experimental unilateral chewing. 目的研究Ⅲ型胶原和纤维连接蛋白基因在偏侧咀嚼大鼠磨牙牙周膜表达中的变化,探讨偏侧咀嚼对牙周膜改建的分子生物学机理。
Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively. 方法:从13d鸡胚骨骼肌mRNAs经RTPCR方法扩增RTEF1的编码序列(cDNA),将其克隆到pGEM和pUC载体并进行核苷酸序列分析。
Objective To observe the effects of iodine deficiency and iodine excess on the expression of rat myocardial myosin heavy-chain ( MHC) mRNAs. 目的观察碘缺乏和碘过量对大鼠心肌肌球蛋白重链(MHC)基因表达的影响。
However, neither ConA nor SEA could induce IFN γ or IL 4 mRNA transcription in 10 and 12 week infected mice indicating the modulated expression of mRNAs of these two lymphokines. 然而,在感染10周和12周小鼠,无论ConA或SEA都不能诱导IFN-γ或IL-4mRNA转录,显示这两种淋巴因子表达受到抑制。
In cardiac cytoplasm, existing n mRNA chain complex's mRNAs quickly translated proteins and the body can transfer speedy and comparatively serious hypoxia information. 心肌等胞质中已有NmRNA链状复合体中mRNA迅速翻译出蛋白质,心肌等组织可传导较严重快速缺氧信息;
By now ten interacting proteins of FMRP have been found. It shows that FMRP involves in transporting specific mRNAs and regulating its translation, also it can modulate dendrite postsynaptic proteins synthesis and dendrite spines maturation. 现已鉴定出十种与FMRP相互作用的蛋白,并发现FMRP能运载特定的mRNAs,调控mRNAs的翻译;FMRP还参与调节树突突触后蛋白的合成和树突棘的成熟。
The expressions of mRNAs and proteins involved in main functions of normal liver such as metabolism, detoxification and biosynthesis could be detected in IHH. 体外分析表明:IHH具有正常肝细胞的主要功能,表达与肝脏代谢、解毒、合成相关的基因和蛋白。
Realtime PCR, Western blot, and immunohistochemistry etc were used to verify the different expression of mRNAs and proteins in two groups. Furthermore, the correlations between the differential proteins and histological grading, pathological staging, prognosis of CRC were also analysed. 应用Realtime-PCR、westernblot和免疫组化等方法在转录和蛋白质水平对其表达差异进行验证,并分析其与结直肠癌组织学分级、临床分期和预后等的关联性。
In situ hybridization results showed that the positive signals for Frizzled 2 were highly detected in the oocyte and stroma in proestrus stage, while moderate or weak Frizzled 2 mRNAs were localized in the oocyte, granulose cells, stroma and corpus luteum from estrus to diestrus. 原位杂交结果表明:动情前期卵母细胞和基质中Frizzled2信号强;而后三个时期的卵母细胞、颗粒细胞、基质和黄体中具有中等或微弱信号。