Comparison of Gas Chromatography-Mass Spectrometry Analysis in Different Extract Parts of Marchantia convoluta and Study on Partial Biologic Activity 拳卷地钱不同提取部位的气相色谱-质谱分析比较和部分生物活性研究
Objective: The methods of silica column chromatography, RP HPLC, UV, IR and LC ESI MS for isolating, purifying and identifying apigenin 7 O β D glucuronide in Marchantia convoluta was established in this article. 目的:建立分离纯化鉴定拳卷地钱中芹菜素-7-O-β-D-葡萄糖醛酸苷的硅胶柱色谱/RP-HPLC/LC-ESI-MS方法。
The analysis method of different extract parts of Marchantia convoluta by gas chromatography-mass spectrometry ( GC/ MS) was established because of the different partial biologic activity in different parts of M. convoluta. 基于拳卷地钱不同部位生物活性的不同,建立了采用气相色谱-质谱(GC/MS)技术分析拳卷地钱不同提取部位的方法。
Methods: The crude extracts of Marchantia convoluta leaves was obtained after Marchantia convoluta leaves were extracted with 80% ethanol and the extracts were concentrated under reduced pressure. 方法:拳卷地钱叶经过80%乙醇提取后,减压蒸馏,得粗提物。
Isolation, Purification and Identification of apigenin-7-O-β-D glucuronide in Marchantia convoluta with Silica Column Chromatography, RP-HPLC and LC-ESI-MS 硅胶柱色谱/RP-HPLC/LC-ESI-MS分离纯化鉴定拳卷地钱中芹菜素-7-O-β-D-葡萄糖醛酸苷
Extraction of Volatile Oil from Marchantia Convoluta by CO_2 Supercritical Fluid Extraction and Analysis by GC-MS 拳卷地钱挥发油的CO2超临界流体萃取及GC-MS分析