Results High and intermediate doses of koumine showed remarkable inhibitory effect on mouse vaginal epithelial cell mitosis and promoted the formation of epidermal granular layer in the scales at the mouse tail. 结果Kou中、高剂量组能显著抑制阴道上皮细胞有丝分裂、促进鼠尾鳞片表皮颗粒层的生成;
Inhibitory effects of koumine on splenocyte proliferation and humoral immune response in mice 钩吻素子对小鼠脾细胞增殖反应及体液免疫反应的抑制作用
Effect of koumine on proliferation of murine CD4~+ T cells purified by magnetic-activated cell sorting in vitro 钩吻素子对免疫磁珠分离纯化的小鼠CD4~+T细胞体外增殖的影响
Objective To assess the separation efficiency of magnetic-activated cell sorting in the purification of CD4+ T cells from murine spleen, and observe the effects of koumine on the proliferation of the separated cells. 目的探讨小鼠脾脏CD4+T细胞的分离方法,观察钩吻素子(Kou)对小鼠脾脏CD4+T细胞体外增殖的影响。
Although gelsemine and koumine both contain an exo-vinyl, an Nb-methyl and most likely a cyclic ether linkage, the structures of the alicyclic parts of these two alkaloids appear also different from each other. 虽然这两种生物碱都含环外乙烯基,Nb-甲基及可能具环醚结构,但是其脂环部分的构造按推断亦不相同。
Conclusion The therapeutic effect of koumine against psoriasis is related to the inhibition of epidermal cell proliferation, promoting the formation of granular cells and decreasing the serum level of IL-2. 结论提示Kou可能通过抑制小鼠表皮细胞增殖与促进其分化并减少炎性因子的生成而发挥作用,为其用于银屑病的治疗提供了实验依据。
Effect of Koumine on Platelet Aggregation in Rabbit 钩吻素子对兔血小板聚集的影响
Results and Conclusion Koumine crystals were isolated and purified from Gelsemium alkaloids effectively. 结果从钩吻中分离得到了无色棱柱状钩吻素子晶体。结论本实验所采用的提取钩吻总生物碱及分离钩吻素子的方法行之有效。
Extraction and separation of koumine from Gelsemium alkaloids On Toxic Mechanism of Gelsemium Total Alkaloids 钩吻总生物碱中钩吻素子的提取与分离钩吻碱毒性作用机理的研究
Crystal and molecular structure of Koumine 钩吻素子的晶体及分子结构
Koumine inhibits the proliferation of mouse spleen lymphocytes. 钩吻素子体外可抑制小鼠脾淋巴细胞的增殖。
Extraction and Separation of Koumine and Experimental Therapy on Psoriasis 钩吻素子的提取分离及对银屑病的实验治疗
Koumine significantly inhibits the proliferation of murine CD4+ T cells due to its immunosuppressive effect and inhibition of IL-2 secretion. Kou明显抑制小鼠CD4+T淋巴细胞增殖反应,此抑制作用可能与Kou抑制小鼠CD4+T细胞IL-2的分泌及免疫抑制作用相关。
Koumine as the highest alkaloid content Gelsemium plants, as the object of study, pigs, sheep and mouse liver microsomal comparative study of their metabolic characterization revealed part of its liver detoxification mechanisms. 钩吻素子作为钩吻植株中含量最高的生物碱,选择其做为研究对象,进行猪、羊和小鼠肝脏微粒体对其代谢表征的比较研究,可以初步揭示其肝脏解毒的部分机制所在。