Aim To proliferate and identify dendritic cells from the peripheral blood of patients with chronic hepatitis B.Methods Peripheral blood mononuclear cells ( PBMC) were isolated from normal donors and patients by centrifugation in Ficoll Hypaque. 目的尝试从慢性乙型肝炎患者外周血扩增培养出大量树突细胞,为进一步临床应用树突细胞治疗慢性乙型肝炎提供物质基础。