Human growth inhibitory factor is a brain-specific metallothionein ( MT3) and a hexapeptide insertion is present in its C-terminal of α-domain. This insertion comprises several glutamate residues, which are reported to be necessary for protection against the cytotoxicity. 人类神经生长抑制因子(GIF)又称MT3,其α结构域存在一个特殊的插入六肽,其中含有多个谷氨酸残基,这些谷氨酸残基被认为与GIF具有保护神经元活性有关。
Cysteine peptides ⅱ. an improved synthesis of protected hexapeptide with the amino acid sequence 6& 11 of the A-chain of insulin and its analogues 含半胱氨酸多肽的研究Ⅱ.胰岛素A链中小环部份(A6A(11))合成方法的改进及其类似物的合成
We used Chinese Hamster Ovary Cells over expressing insulin receptors ( CHO IR) as screening targets to select peptide ligands of insulin receptors from a phage display random hexapeptide library. 以高效表达胰岛素受体的中国仓鼠卵巢细胞(CHO-IR)为筛选靶标,经过一轮酸洗脱和三轮特异性竞争洗脱筛选,成功地富集了与胰岛素受体结合的重组噬菌体克隆。
Methods: ① A phage-displayed random hexapeptide library was screened with PSA as target. 方法:①用豌豆凝集素(PSA)作为靶蛋白,对噬菌体展示的随机六肽库进行亲和筛选;