Objective: Oxidative stress effect induced by cooking oil fume volatile organic compounds ( COF VOCs) was studied on human embryonic diploid lung fibroblasts ( HELF). 目的:探讨烹调油烟挥发性有机物对人胚肺成纤维细胞(HELF)的氧化应激效应。
Objective To observe the expression of HO-1, MT1A, MT2A in human embryonic lung fibroblasts ( HELF) induced by supernatant of alveolar macrophage ( AM) exposed to silica. 目的观察染尘巨噬细胞上清液对人胚肺成纤维细胞(HELF)血红素氧合酶-1(HO-1)、金属硫蛋白(MT)表达的影响。
Objective To investigate the relationship between the hormesis of proliferation and oxidative stress induced by sodium arsenite ( Na_2AsO_2) in human embryo lung fibroblasts ( HELF). 目的探讨亚砷酸钠(Na2AsO2)所致人胚肺成纤维细胞(HELF)增殖兴奋效应与氧化应激的关系。
Objective To construct a recombinant eukaryotic vector bearing the fusion gene of human LIF gene and EGFP ( enhanced green fluorescent protein), and to transfect it into human embryonic lung fibroblasts ( hELF). 目的:构建携带人LIF基因与增强型绿色荧光蛋白(EGFP)的融合表达载体,并转染人胚肺成纤维细胞,为研究LIF在维持人胚胎生殖细胞自我更新中的作用机制奠定基础。
Comparison between STO cells and hELF used as feeder layers of embryonic germ cells 以STO细胞和hELF作为胚胎生殖细胞培养饲养层的比较
( CONCLUSION:) HELF as feeder layers is better than STO cells in culturing EG cells. 结论:在培养人EG细胞时,hELF饲养层较STO细胞饲养层更有优势。
Conclusion The sodium arsenite can induce the hormesis of proliferation in HELF with the dose-effect relation of an inverted U curve. The mechanisms probably relates to different levels of oxidative stress induced by sodium arsenite of different concentrations. 结论Na2AsO2可引起HELF细胞增殖明显兴奋效应,其剂量-反应关系呈倒U形曲线,其机制可能与不同浓度Na2AsO2引起HELF细胞不同程度的氧化应激有关。
Conclusion ATRA could block B ( a) P-induced cell cycle promotion through cyclin D1/ E2F-1 pathway in HELF. 结论ATRA通过cyClinD1/E2F1途径阻断苯并(a)芘诱导的HELF的细胞周期改变。
Conclusion ERK and JNK, but not p38, mediated benzo ( a) pyrene-induced cell cycle changes by AP-1 transactivation in HELF. 结论ERK和JNK通过活化AP-1介导B(a)P诱导的细胞周期改变;而B(a)P诱导的AP-1活力增强及细胞周期改变与p38无关。
Mitotic checkpoint function of HELF cells transfected with mutant gene 转染突变基因对HELF细胞有丝分裂的影响
P21 gene were integrated into the genome DNA of HELF cells by DNA recombination technique to make cell-aging model in the experiments in vitro. This cell-aging model was interfered by TP. 体外实验用基因重组技术将p21基因整合到人胚肺成纤维细胞基因组中,建立细胞衰老模型,并用银耳多糖对此进行干预;