Methods The cellular activity was determined with light microscope technique and cell counting kit8 ( CCK-8) to compare the growth difference of gymnocyte team, chitosan team, collagen ⅰ team and chitosan-collagen multiplicity carrier team. 方法应用光镜技术及CCK-8试剂测定细胞活力来观察比较肝内胆管上皮细胞裸细胞培养法、壳聚糖培养法、Ⅰ型胶原培养法及壳聚糖-胶原复合载体混合培养法的生长情况。