Methods Neutral glycosylsphingolipid was separated from normal human brain by column chromatography with methanol/ chloroform solution for gradient elution, and the resultant underwent 1H-NMR and 13C-NMR analyses for identifying the structure of Glob-N. 方法人脑中性鞘糖脂经过加压柱层析,梯度甲醇/氯仿溶液洗脱。对所得到的产物行1H-NMR和13C-NMR测定。