At present, the main method for glucose-1-phosphate preparation is enzymatic synthesis, and there are free enzyme and immobilized enzyme two ways. 葡萄糖-1-磷酸的制备主要采用酶法合成,且有游离酶法和固定化酶法之分。
For early realization of the industrialization of glucose-1-phosphate and its application, this paper attempts to use microbial direct fermentation methods to produce glucose-1-phosphate. 为了早日实现葡萄糖-1-磷酸的工业化生产及其应用,本论文试图采用微生物直接发酵的方法来生产葡萄糖-1-磷酸。
The experiment screened one strain which can directly produced extracellular glucose-1-phosphate by fermentation; then a mutant strain high-yielding glucose-1-phosphate was obtained by UV mutagenesis; finally the fermentation conditions of the high-yielding strain were optimized, the output of glucose-1-phosphate improved further. 通过试验,筛选出一株能直接发酵产胞外葡萄糖-1-磷酸的菌株,经紫外诱变选育到一株高产菌,最后对高产菌株发酵条件进行了优化,其产量得到了进一步提高。