And then try to exam the immobilization yield of the immobilized enzyme, and the enzyme activities of immobilized enzyme and free enzyme to demonstrate some characters of immobilized enzyme. 然后测定固定化酶的固载率,游离酶和固定化酶的活性,从而确定固定化酶的部分性质。
Compared the effects of environmental factors on the activity of the free enzyme with that of two kinds of immobilized enzymes, the immobilized enzymes were found to increase their resistance to the changes of environment. 比较了环境因素对游离酶和两种固定化酶活性的影响,结果表明青霉素酰化酶固定化后,抗环境变化的能力明显提高。
After dealed with Cu2+, Mn2+ Mg2+, the activity of free enzyme decreased but the activity of immobilized enzyme increased strikingly. C了+、、山2+、M扩作用游离酶和固定化酶后,游离酶活性有不同程度的降低,而固定化酶活性有显著提高。
Compared with the free enzyme, it showed a wider range of reaction temperature and PH value. 与游离酶相比,其最适作用温度范围、pH值范围均比游离酶范围宽;
After being stored at 4 ℃, pH 7.0 for 30 days, the immobilized α-amylase retained 91.6% of its initial activity which was 12.3% more than that of free enzyme. 固定化α-淀粉酶在4℃,pH7.0的缓冲液中保存30d,其活力仍保持最初活力的91.6%,这比其自由酶的高12.3%。
In addition, stability and maneuverability immobilization enzyme are prior to free enzyme. 另外,固定化酶与游离酶相比,稳定性和可操作性都有较大的提高。
Immobilization methods for the free enzyme were also studied. 研究了降解酶的固定化方法。
The enzyme-metal-substrate complex has a conformation which is different from that of free enzyme. PK的活化离子-底物复合物的构象不同于自由酶的构象。
Experimental studies have shown that the stability of the immobilized enzyme was significantly higher than the free enzyme. 通过实验研究表明,固定化酶的稳定性明显高于游离酶。
Compared with free enzyme, the pH and thermal stability of immobilized lactase were increased. 固定化后的乳糖酶的pH,热稳定性都较游离酶有明显提高。
Compared to the free enzyme, immobilized enzyme shows better adaptability to the environment, the higher catalytic activity, and good repeated utilization. 结果显示与自由酶相比,以此材料为载体的固定酶,有更好的环境适应性、更高的催化活性及良好的重复利用性。
Compared with the free enzyme, immobilized enzyme has many advantages, such as great enhanced stability, high efficient catalysis, easy separation of the products and so on. 固定化酶技术与游离酶相比,固定化酶具有很多优点,如稳定性大大增强,催化效率高,易于产物的分离等,因此国内外对胰蛋白酶的固定化作了大量的研究。
Than free enzyme immobilized enzyme is more stable 、 repeatable use and more easily separated from reaction system, thus is widely used in biological catalysis, food industry, environmental restoration and so on. 固定化酶较之游离酶更加稳定、可重复利用、且更加易从反应体系中分离出来,因此被广泛用于生物催化、食品工业、环境修复等领域。
Compared with the free enzyme, immobilized ALDC has higher thermal stability, broader pH stability and better reusability. ( 4) Immobilized ALDC was used in continuous beer fermentation. 与游离酶相比,固定化酶有较高的热稳定性、更宽泛的酸碱稳定性和更好的重复使用性。
Under the optimum immobilization conditions, the pH, operational and storage stabilities of immobilized enzyme are greatly improved compared to the free enzyme, which was beneficial to the reuse and storage of immobilized enzyme. 在最佳固定化条件下制得的固定化酶与游离酶相比,固定化酶的pH稳定性、操作稳定性和储藏稳定性都有了较大改善,有利于木瓜蛋白酶的重复使用。
In order to overcome some disadvantage from the free enzyme catalysis, the free enzyme was immobilized on the carrier. Then, the immobilized lipase was applied in the resolution of 2-chloropropionic acid ester. 为了克服游离酶催化的一些不足之处,将游离酶固定在载体上,寻求最佳的脂肪酶固定化方案,并将固定化酶应用于2-氯丙酸酯的拆分。
Finally, the free enzyme and the nature of the immobilized enzyme comparison. 最后对自由酶和固定化酶的酶学性质进行比较。
At present, the main method for glucose-1-phosphate preparation is enzymatic synthesis, and there are free enzyme and immobilized enzyme two ways. 葡萄糖-1-磷酸的制备主要采用酶法合成,且有游离酶法和固定化酶法之分。
The optimal conditions of enzyme immobilization were mainly investigated by the single factor and the orthogonal experiments. The basic enzymatic properties of immobilized ALDC and free enzyme were researched. 本文以工业树脂为载体对ALDC进行固定化研究,通过单因素和正交试验确定了固定化ALDC的最优条件,研究了固定化酶和游离酶的基本酶学性质。
The immobilized lipase exhibited excellent thermal stability and reusability in comparison with free enzyme. 与游离酶相比,固定化酶显示了优异的热稳定性和重复使用性。
The results indicated that the immobilized enzyme had higher thermal and storage stability than the free enzyme. 比较了游离酶和固定化酶的热稳定性和储存稳定性,结果表明,固定化酶的热稳定性和储存稳定性远远好于游离酶。
Free enzyme can be used only once, on the contrary, immobilized enzyme used as biocatalyst can be utilized repeatedly, and what is more, it has the advantage of stable activity, simple operation and facility to separation and purification for production. 但游离酶只能使用一次即抛弃,而固定化酶作为生物催化剂可多次重复利用、稳定性、操作简单和产物易于分离纯化等优点,因此对其进行固定化研究具有重要意义。
Optimum temperature, thermal stabilities of immobilized enzyme were compared with the free enzyme. The immobilized enzyme were reused. 通过比较游离酶与固定化酶的最适温度和热稳定性等性质,研究了固定化酶的重复使用率。
Since enzyme is a protein in chemical nature, use of free enzyme will introduce a new source of contaminate into the extraction. Herein, immobilized enzyme can solve the problem very well for its superiority over free enzyme. 由于酶的化学本质是蛋白质,游离酶在参与酶解特定大分子底物的过程中也同时对烟用香精香料产生污染,固定化酶在这方面的优越性可以很好解决这一矛盾。