He allowed these "enucleated" eggs to be fertilized by sperm from sea urchins of the genus echinus. 他让这些“无核”的卵跟海胆属的海胆的精子受精。
Enucleated cells are prepared by removing cell nuclei in the presence of the drug, cytochalasin B. 无核细胞是通过药物、细胞松弛素b把细胞核去掉制备的。
The retinal sections were stained with histochemical and immunofluorescent staining and examined by confocal microscopy 1,3,7 and 28 days after injection. eye enucleated, and retinal sections studied by histochemistry, immunofluorescence labeling, and confocal microscopy. 分别于手术后1、3、7和28d摘除眼球,视网膜切片进行组织化学、免疫荧光染色,共聚焦显微镜检查。
A preliminary study on the nuclear transfer of bovine blastomeres into enucleated buffalo oocytes 黄牛和水牛种间核移植研究初报
The reproduction of infectious swine vesicular disease virus in enucleated cells 猪传染性水泡病病毒在去核细胞内的复制
The assembly of microfilament was also inhibited by CB. The combined effects weaken the connections between nucleus and cytoplasm, which facilitate the nucleus to be enucleated. 细胞在CB的作用下,微丝聚集受到抑制,细胞核与细胞质间的连接变得松散。
The scientific intension of concept of micro and macro applied to hydrocarbon generation and accumulation from organic matter is enucleated in terms of Lagrange function; 应用拉格朗日(Lagrange)函数阐明了微观与宏观的概念在有机质成烃和成藏中的科学内涵;
The fuse rate of rabbit 32-cell stage embryo blastomere and mouse enucleated M ⅱ oocytes were 66%. 兔32-细胞期胚胎卵裂球与小鼠去核的MⅡ期卵母细胞融合率为66%。
Spindle-View, a computer-aided optical apparatus specific for oocyte manual, was used to examine the different locations of spindle in mice oocytes at various ages to analyze the relationship between the spindle position and the enucleation efficiency of mice oocytes which were enucleated under the Spindle-View. 探讨小鼠不同卵龄卵母细胞纺锤体位置与去核效率之间的关系。用Spindle-View观察小鼠不同卵龄卵母细胞纺锤体位置,然后对其进行去核操作。
A DC pulse of 1200v/ cm and 80 μ s was applied to induce membrane fusion between a blastomere and an enucleated oocyte. 对组合卵施加一个1200伏/cm(V/cm)和80微秒(μs)的直流电脉冲,使卵裂球的细胞核导入去核卵母细胞之中。
The micro and ultrastructure, which were taken respectively from fluorescent staining by binding of DAPI dye to DNA and TEM, showed that exogenous lambda DNA induced nuclear reconstitution in the enucleated eggs. DNA荧光染色显微观察和超微结构研究表明,在去核卵细胞中,外源LambdaDNA可诱发构建细胞核。
In this experiment, we studied porcine cloning by transplantation of cumulus cell and cultured granulosa cell nuclei into enucleated in vitro matured oocytes, and some issues related to it, such as oocyte activation, cell fusion and oocyte maturation. 本实验对猪体细胞核移植及其相关问题如细胞融合、卵母细胞激活和卵母细胞体外成熟进行了研究。
Finally, the important significance of integrated science education and the difficulties during practice were enucleated. 最后,阐明实施综合理科教育的重要意义及现行遇到的困难。
[ Conclusion] Mouse reconstructed embryos with 1 polar body and 2 pronuclei can be formed after transferring cumulus cell nucleus and spermatozoa into enucleated MII oocyte. 【结论】将小鼠卵丘细胞核、精子同时移植到小鼠的体内成熟卵浆中,可以诱导极体排出,形成2原核+1极体的重构胚胎。
This phenomenon was also found in enucleated cells. 在脱核细胞中,表面受体聚集现象与在完整细胞中一样。
This experiment was conducted to study enucleated method, which is one of the key techniques for animal cloning. 本试验对克隆技术中关键步骤之一的去核方法作了深入研究。
The blastomeres separated from the embryos were injected into the perivitelline space of enucleated secondary oocytes under the control of micromanipulator. 在显微操作仪的控制下,将分离后的单个卵裂球注入去核次级卵母细胞的卵周隙。
The eyes were enucleated at different time ( 1, 2, 3,4 and 8 weeks) after operation and observed the change of retinal structure by light and transmission electron microscopy. 术后分别于1周、2周、3周、4周及8周观察两实验组眼底及视网膜结构的光镜及电镜的变化。
Further, the enucleating rate of oocytes being enucleated by point-hit method was 90.0%, significantly higher than that by sucking method ( 68.3%, P < 0.01), not different with that by squeezing method ( 88.3%). 而且,点击去核法去核率为90.0%,极显著地高于吸引法的68.3%(P<0.01),与挤压法去核率88.3%差异不显著;
Cloned embryos were reconstructed by nuclear transfer technique with fibroblasts and embryonic stem ( ES) cells of Kunming albino mouse taken as nuclear donor, enucleated MII oocytes of Kunming albino mouse and Japan big-eared white rabbits taken as recipient oocytes. 以昆明白小鼠成纤维细胞和胚胎干(ES)细胞作为供核细胞,以昆明白小鼠和日本大耳白兔的MII期去核卵母细胞作为受体,采用核移植方法,构建了克隆胚胎。
Preactivating donor cells and enucleated oocytes with ionomycin has no significant enhancive effect on the cleavage of constructed embryos, but it arrests the blastocyst development. 用离子霉素或乙醇预激活去核卵母细胞,对重组胚的卵裂和发育无明显影响。
Methods 117 specimens of enucleated eyeball were observed by light microscopy, among which 18 cases of corneal fissure after ocular contusion, 99 cases of corneal perforation. 方法对因角膜外伤后摘除眼球标本117例进行光镜观察,其中因眼挫伤致角膜裂伤18例,角膜穿孔伤99例。
Meanwhile, the operate steps of TBT has be enucleated, the areas which TBT applied to should be limited. 阐明了运用TBT模式进行项目融资的操作步骤,界定了TBT项目融资模式的应用范围。
Rabbit nuclear transplant ( NT) embryos were produced from enucleated oocytes and blastomeres of different developmental stage embryos by micromanipulation and electrofusion. 将发育不同时期的兔胚和移核胚的卵裂球细胞核与去核的成熟卵母细胞共同组成移核胚,通过中间受体培养和移植实验检验胚胎的发育能力。
Nuclear transplanted embryos produced by transferring blastomeres from 4-32 cell embryos into enucleated secondary oocytes can develop into normal kids in vivo; 由山羊4-32细胞期胚胎的卵裂球和去核次级卵母细胞构成的卵核移植胚在体内仍可发育为正常个体;
The horse fibroblasts were used as donor of nuclear to reconstruct cloned embryos with enucleated bovine oocytes. 马成纤维细胞与牛卵母细胞构建克隆胚。
It has been demonstrated that the mouse oocytes can be enucleated accurately with the treatment of appropriate sucrose. 研究表明用适当浓度的蔗糖处理小鼠卵母细胞能准确去除细胞核。
The somatic cell nuclear transfer of pigs is injecting donor cells into enucleated oocytes, make the donor nucleus carrying on normal reprogramming through the auxiliary activation, then transplant the embryo into acceptor animal, which process of cloned offspring. 猪体细胞核移植,就是将供体细胞注入已去核的卵母细胞内,通过辅助激活等手段使供体细胞核进行重编程,然后对重组胚胎进行胚胎移植,从而得到克隆后代的过程。