emsa
英
美
网络 爱慕莎; 凝胶阻滞; 凝胶迁移; 凝胶电泳迁移率分析; 电泳迁移率改变试验
复数：emsas
双语例句
- DNA binding proteins that bind specifically to three probes of positive footprinting area of CEA promoter were detected by electrophoretic mobility shift assay ( EMSA).
利用电泳迁移分析法（EMSA）检测这些细胞株与CEA启动子阳性足迹区探针结合的DNA结合蛋白。 - First, we carried out Electrophoretic Mobility Shift Assay ( EMSA) to investigate the binding difference of SNPs and transcription factors.
首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。 - Activation of activator protein-1 ( AP-1) was measured by electrophoretic mobility shift assay ( EMSA).
电泳迁移率变动分析法（EMSA）研究核转录因子SP-1的活性。 - Methods The NF-κ B activity in monocytes was measured by electrophoretic mobility shift assays ( EMSA) and the level of TNF-α in serum was measured by radioimmunoassay.
方法以电泳迁移率变动分析法（EMSA）检测外周血单个核细胞的NFκB活性，放射免疫法测定血清TNFα水平。 - Electrophoretic mobility shift assay ( EMSA) was used to detect the NF-κ B/ DNA binding activity.
用凝胶电泳迁移率改变试验（EMSA）检测肺动脉NFκB/DNA结合活性。 - NF κ B DNA binding activity was evaluated with electrophoretic mobility shift assay ( EMSA).
采用：（1）凝胶电泳迁移率改变分析检测核因子（NF）κbDNA结合活性； - The DNA biding activity of AP-1 was analyzed by electrophoretic gel mobility shift assay ( EMSA).
电泳迁移率变更分析（EMSA）检测AP1DNA结合活性的变化； - Changes in DNA binding activity of CREB and the concentration of cAMP and cGMP in mouse thymocytes were respectively assayed by electrophoresis mobility shift assay ( EMSA) and competitive protein binding assay.
采用电泳移动变化分析及竞争性蛋白结合分析法分别检测了胸腺细胞内CREB的DNA结合活性和cAMP、cGMP含量的变化。 - Electrophoretic mobility shift assay ( EMSA) confirmed that transcription factor Sp1/ Sp3 could bind GC ⅰ, GC ⅱ and GC ⅳ and that the retarded bands were competed by Sp1 and Sp3 antibodies.
电泳迁移率变动分析（EMSA）证实，GCⅠ，Ⅱ，Ⅳ能与转录因子Sp1和Sp3结合，而且结合条带可被转录因子Sp1和Sp3的抗体所拮抗。 - Determination of HIF-1 α Binding Activity by EMSA in Pulmonary Arteries of Rats With Hypoxia-induced Pulmonary Hypertension
HIF-1α结合活性及蛋白含量的表达变化在缺氧性肺动脉高压大鼠中的作用和意义 - The E2F1 DNA binding activities were measured by gel mobility shift assay ( EMSA).
用凝胶迁移率变动分析法（EMSA）检测其与DNA的结合活性。 - The activity of NF-κ B and the concentration of TNF-α and IL-10 in the supernatant were measured by electrophoretic mobility shift assay ( EMSA) and radioimmunoassay ( RIA) respectively.
用凝胶电泳迁移率改变分析（EMSA）法和放射免疫法分别测定各组核提取物中NFκB活性和细胞培养上清中TNFα，IL10含量。 - The method for detection of NF-κ B-DNA binding activity in the aorta of three groups was electrophoretic mobility shift assay ( EMSA).
实验结束时，用电泳移动迁移技术（EMSA）检测三组兔主动脉组织中NF-κB-DNA结合活性； - EMSA analyses of transcription factors, C/ EBP and HMG-like protein in human lung cancer cells
人肺癌细胞转录因子&C/EBP和HMG样蛋白的EMSA检测 - Western blot and EMSA ( electrophoretic mobility shift assay) demonstrate that the natural and denatured Cdx-2s from different species ( mouse and human) can be detected by the prepared anti-Cdx-2 antibody.
最后，Western印迹和EMSA实验分析结果表明，该抗体可用以检测不同种族（人和鼠）的、变性或非变性的Cdx2，并显示有较好的抗Cdx2的专一性和较高的灵敏度。 - Transcriptional and DNA binding activity were analyzed by reporter gene assay and electrophoretic mobility shift assay ( EMSA) respectively. Western blot was used to determine the protein expression;
运用报告基因法和凝胶电泳迁移率法（EMSA）分析AP1和NFκB反式激活活性和DNA结合活性，蛋白质印迹检测蛋白质表达； - Methods Chemiluminescent electrophoretic mobility shift assay ( EMSA) was used to detect the activity of NF-κ B in splenic artery and vein. The expression of TNF-α mRNA in splenic artery and vein of PHT patients and normal vessels was detected by RT-PCR analysis.
方法采用化学发光凝胶电泳迁移率实验（EMSA）方法检测肝硬化PHT患者脾脏动、静脉和正常血管NF-κB的活性，用逆转录-聚合酶链反应（RT-PCR）方法检测TNF-αmRNA的表达情况。 - Meanwhile, pulmonary STAT-1 DNA-binding activity was detected by electrophoretic mobility shift assay ( EMSA).
凝胶电泳阻滞分析（EMSA）测定STAT-1的DNA结合活性； - Activities of DNA-binding of nuclear factor-kappa B ( NF-κ B) and activator protein-1 ( AP-1) were measured by electrophoretic mobility shift assay ( EMSA).
应用凝胶电泳迁移率试验（EMSA）测定核因子-κB（MF-κB）和活化蛋白-1（AP-1）的DNA结合活性。 - Objective: To establish a fast and simple method of electrophoretic mobility shift assay ( EMSA) for the identification of DNA-binding protein nuclear factor-kappa B ( NF-κ B).
目的：建立一种快速、简化的电泳迁移率变动分析（EMSA）方法检测DNA结合蛋白核因子-κB（NF-κB）。 - Methods Electrophoretic mobility shift assay ( EMSA) was employed to determine STAT-DNA binding activity.
方法采用凝胶阻滞电泳（EMSA）测定DNA-STAT结合活性变化。 - Methods RIA, Western blot and EMSA methods were used to detect serum concentration of cortisol, expression of glucocorticoid receptor ( GR) and glucocorticoid response element ( GRE) binding efficiency in brain tissue.
方法采用放免测定、westernblot和EMSA方法检测两株小鼠即时应激前后血清皮质醇浓度、脑组织糖皮质激素受体（GR）表达和脑组织GRE结合效率。 - Furthermore, Western blot and gel electrophoresis mobility shift assay ( EMSA) were performed to detect the mechanism of PLC γ 1 in colorectal cancer cell adhesion to matrix.
PLCγ1在结直肠癌细胞中作用的信号转导机制采用蛋白质印迹及凝胶迁移率变动分析（EMSA）。 - TESTING The activation of NF-AT in T Lymphocyte of EAU was assayed by using electrophoretic mobility shift assay ( EMSA).
采用电泳迁移率改变试验对EAUT淋巴细胞NF-AT活性检测。 - Transcription factor c-myb activity was confirmed by electrophoretic mobility shift assays ( EMSA) in the two cell lines.
用凝胶电泳迁移率变动分析（EMSA）验证有活性差异的转录因子c-myb。 - By luciferase reporter assay and electrophoretic mobility shift assay ( EMSA), we confirmed that ISRE I and ISRE II are the molecular basis of RIG-G gene transcription.
通过启动子报告基因实验以及EMSA（Electrophoreticmobilityshiftassay）实验等，我们证实RIG-G基因启动子区的ISREI和ISREII两个顺式作用元件是该基因转录表达的分子基础。 - The promoter regions of these genes were PCR amplified, and then EMSA was carried out to study the binding of the purified AlmR ⅰ protein to these regions.
利用PCR扩增得到了这些基因的启动子区，然后通过凝胶阻滞实验对纯化的AlmRⅠ蛋白与所扩增片段的结合进行了研究。 - By the EMSA test, the MYC cis-acting element could bind protein and then induce the activity of promoter. But whether this cold-response regulation is similar to Arabidopsis thaliana or not is still to be further studied.
通过EMSA试验初步验证，枳中MYC顺式作用元件能与蛋白结合，诱导启动子的活性，但是此低温应答调控方式与拟南芥中反应通路是否一致，还有待进一步的研究。 - Then, we verify this conclusion again with the ChIP and EMSA assays.
随后，我们使用ChIP实验和EMSA实验验证了此结论。