Electroporated sperm mediation of an "whole fish" lysozyme gene construct into Scophthalmus maximus 转全鱼溶菌酶基因大菱鲆的研究
The dendritic cells ( DCs) were generated from HSCT donors in vitro, and its immunologic activity was studied by using flow cytometry and mix lymphocyte reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic acid vaccine. 体外培养移植供者树突状细胞,用流式细胞术、混合淋巴细胞反应检测其免疫活性,通过电转法将HA-1基因转染树突状细胞,构建树突状细胞核酸疫苗。
The above vectors were respectively electroporated into T7 recombination strains and obtained four lipase expression strains. 通过电转化将表达质粒转入T7重组菌获得4种脂肪酶工程菌。