The lowest and essential medium ( Dulbecco, DEME, GIBCO company); 最低必需培养液(Dulbecco,DEME),Gibco公司;
Methods: CD34~+ cells from naturally aborted human fetal liver were isolated with MACS Kit, and cultured in Dulbecco's modified Eagle's medium ( DMEM) supplemented with 10% fetal bovine serum ( FBS). 方法:采用MACS试剂盒分离人胚胎肝CD34+细胞,以DMEM+10%胎牛血清培养液培养;
Methods The rabbit thyroid chondrocytes were isolated and plated into 4 groups of Dulbecco modified Eagle medium: group 1, Dulbecco modified Eagle medium ( DMEM) only; 方法分离兔甲状软骨细胞,分4组培养,每组用含不同成分的培养基。第1组:只用DMEM培养基;
Objective: To explore the effect of cox-2 on liver metastasis of colon cancer in mice by studying the correlation between cox-2 and angiogenesis factor, VEGF 、 FGF-2. Methods: Colon cancer cell strain, HT-29 and HCT-116 were cultured in Dulbecco modified Eagle medium. 目的:研究环氧合酶-2及其选择性抑制剂塞来昔布和血管生成因子VEGF、FGF2关系,从而探讨环氧合酶-2对结肠癌肝转移瘤血管形成的影响。
The same bulk of Eagle medium culture medium improved by Dulbecco without blood serum was added in control group. 对照组加相同容积的无血清Dulbecco改良的Eagle培养基培养液。