A amplified fragment length polymorphism ( AFLP) molecular marker system on B. tabaci was funded by adjusting the DNA density, Mg~ 2+ density, dosage of dNTP and other reactions parameter etc. 通过DNA模板浓度、Mg~(2+)浓度、dNTP用量以及其它反应参数等的摸索、调整,建立了一套完善的烟粉虱AFLP分子标记技术体系。
One mechanism identified for inhibition of DNA repair or replication by p21 is depletion of dNTP pools. P21介导DNA修复和复制的抑制机制之一是通过耗竭dNTP库。
Third, assure quality and amount of mRNA, reduce amount of dNTP, select of suitable temperature of inverse transcription and PCR renaturation ( the primers to anneal to the template DNA strands), the methods can optimize parameter of PCR. 3保证起始(模板)mRNA质量和浓度,降低dNTP浓度,选择最适的反转录和PCR退火温度,可优化PCR参数。
Study About the Relationship of dNTP, Mg~ ( 2+) and Multiplex Polymerase Chain Reaction 在多基因PCR中对dNTP与Mg~(2+)的浓度关系的研究
[ METHOD] Different levels on concentration of DNA template, primer, dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation. All factor influence for SRAP-PCR in peanut genome was investigated. 【方法】对模板DNA、引物、dNTPmixture、taqDNA聚合酶浓度及退火温度设置不同梯度,研究各因素对PCR结果的影响;
Moreover, the concentrations of Mg2+, dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR. 在等位基因特异PCR中,Mg2+、dNTP及taqDNA聚合酶的用量均大于普通PCR。
After testing on the annealing temperature and the concentration of primer, dNTP and MgCl_2, a suitable PCR system was established. 在对引物、dNTP、MgCl2的浓度及退火温度等参数进行测试后,建立了合适的PCR反应体系。
The results indicated that the concentration of primers, the annealing temperature and the number of cycles have more effect on the efficiency of multi-PCR, but the concentration of dNTP, the concentration of Mg~ ( 2+) and the content of Taq have fewer. 试验结果表明,引物浓度、退火温度和循环次数对该反应体系影响较大,而dNTP浓度、Taq酶含量以及Mg2+浓度的影响较小。
The procedure of DDRT-PCR applicable for silver staining was optimized by adjusting the amount of several critical reagents, including total RNA, anchor primer, arbitrary primer, cDNA and dNTP. 通过调整DDRT-PCR中总RNA、锚定引物、随机引物、cDNA和dNTP等关键试剂的用量,优化了适用于银染检测的DDRT-PCR方法。
It also showed that the PCR reaction residues ( including dNTP, primers, and metal ion) affected badly on the sequencing quality, so the purification of PCR products was necessary before sequencing. PCR反应体系残留混合物(dNTP、引物和盐离子等)对其测序质量有明显不利影响,PCR产物纯化后其测序质量能明显提高;
With orthogonal experiment, this paper studied the effects of the concentrations of Mg~ ( 2+), dNTP and primer, the anneal temperature, and the extending time and cycling times on RAPD of soil microbes. 采用正交实验设计,对影响土壤微生物RAPD扩增体系的Mg2+、dNTP浓度及引物浓度进行了研究,同时对退火温度、延伸时间及循环次数进行摸索。
Objective: To investigate and compare the effects of deoxynucleoside triphosphates ( dNTP) on human lymphocytes and plant root tip cells SCE. 目的:研究和比较4种三磷酸脱氧核苷(dNTP)对人外周血淋巴细胞和植物根尖细胞姐妹染色单体交换(SCE)的影响,探讨其发生的机理。
Concentration of primer, magnesium chloride, dNTP, template DNA, Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment. PCR反应体系中不同模板含量、引物浓度、taqDNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。
Screening ISSR-PCR the template concentration, primer concentration, dNTP concentration and Taq enzyme dosage, optimizing ISSR-PCR system. 4. 分别筛选ISSR-PCR扩增的模板浓度、Taq酶用量、引物浓度及dNTP浓度,优化ISSR-PCR扩增体系。
In the absence of target DNA, complementary DNA showed the status of the single-stranded, RCA reaction was initiated by addition of the circular template, polymerase, ligase and dNTP. 在没有目标物DNA时,互补DNA呈现单链状态,其在环状模板,聚合酶,连接酶以及dNTP的存在下进行RCA扩增。
One of the mechanisms causing gene mutation might be to change of the Taq DNA polymerase conformation. It interfers with the enzyme-substrate-dNTP binding site and further causes replication mismatch. 这一实验结果也说明了稀土是一种诱变剂,引起基因突变的机制之一可能是改变taqDNA聚合酶的立体构象,干扰了酶与底物dNTP结合位点,从而引起复制错配。
Strand-displacement amplification is a sensitive, rapid and specific isothermal DNA amplification method. But presence of dNTP [ α S] in the reaction limits the application of SDA in molecular biology. 链置换扩增技术是一种快速、灵敏、特异的DNA等温扩增方法,但硫修饰的dNTP的掺入限制了其在分子生物学领域的应用范围。