We observed apoptosis with terminal transferase deoxyuridine nick-end assays and tested NO of the prostate tissue with nitrite reduction test then calculated the activity of NOS. 通过原位末端检测技术测定前列腺组织细胞凋亡情况;应用亚硝酸盐还原酶法检测前列腺组织中NO变化并计算NOS活力的变化;
Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas ( TDT) mediated deoxyuridine triphosphate ( dUTP) biotin nick ending labeling ( TUNEL); 用DNA缺口原位末端标记(TUNEL)技术检测细胞凋亡;
Methods: Apoptotic spermatogenic cells were examined by Wright Giemsa staining and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate ( dUTP) biotin nick end labeling ( TUNEL) technique. 方法:采用瑞-姬染色和末端脱氧核苷酸转移酶(TdT)介导的脱氧核苷酸原位末端标记法(TUNEL)检测凋亡生精细胞。
Histopathological rejection grading in the grafted tissue was performed with hematoxylin eosin ( HE) staining. Apoptosis cells were detected by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling ( TUNEL), and the apoptosis index ( AI) was calculated. 苏木精-伊红(HE)染色进行移植组织排斥反应的病理分级,原位末端标记(TUNEL)法检测移植组织细胞的凋亡情况,计算凋亡指数。
The contents of NO and NOS were measured histochemically. Apoptosis of myocardium was detected for apoptosis index ( AI) as the index of ischemic reperfusion injury with in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling ( TUNEL). 应用组织化学方法测定心肌组织中NO、NOS的含量,应用核酸原位末端标记法(TUNEL)测定心肌细胞凋亡指数,作为评价心肌缺血再灌注损伤的指标。