Cassava denaturant starch is mainly used for instant noodles, ham, fish balls, meat sauce, tomato sauce and other industries. 木薯变性淀粉主要用于方便面、火腿鱼丸、肉酱、蕃茄酱等行业。
Studies on unfolding of lysozyme Induced by guanidine hydrochloride at different macromolecular crowding Unfolding and Refolding of Bacillus Amyloliquefaciens α-amylase Induced by Denaturant 大分子拥挤环境中溶菌酶的去折叠过程变性剂诱导的淀粉液化芽孢杆菌α-淀粉酶去折叠过程和重折叠过程的研究
A light volatile flammable poisonous liquid alcohol; used as an antifreeze and solvent and fuel and as a denaturant for ethyl alcohol. 一种淡的挥发性、易燃、有毒液态醇,用作防冻剂、溶剂、燃料和乙醇变性剂。
Therefore, denaturant plus SDS lysis could be an efficient and reliable method to extract DNA in molecular ecology studies. 因此,变性剂加SDS高盐法是一种更为高效、可靠且适合于环境微生物分子生态学研究的DNA提取方法。
Clinical application of non denaturant polyacrylamide gel electrophoresis and silver staining test 非变性聚丙烯酰氨凝胶电泳及银染法临床应用探讨
Denaturant gradient gel electrophoresis in the genetic diagnosis of hereditary multiple exostoses 变性梯度凝胶电泳在遗传性多发性外生性骨疣基因诊断中的应用
Spectroscopic study on glucose isomerase affected by denaturant 变性剂对葡萄糖异构酶影响的光谱研究
Effect of sodium dodecyl sulfate ( SDS) denaturant on activity and conformation of alkaline phosphatase from Haliotis diversicolor SDS变性剂对杂色鲍碱性磷酸酶的活力与构象的影响
As a denaturant for protein, SDS not only makes the HRP lose its activity because of denaturalization, but also decrease the Michaelis constant and the maximum velocity of the reaction for the substrate OPD. SDS作为一种蛋白质变性剂,不仅能够使HRP变性失去部分活性,而且还能使HRP对底物OPD的米氏常数Km(OPD)和最大反应速率都比其在缓冲体系中小;
Through modification of the protocol of our lab, a new total RNA isolation method for potato tubers was established by changing the content of denaturant. 通过改进前人的变性液配方,配合本实验室的抽提方法,建立了一个改良的适用于多淀粉块茎、块根组织RNA抽提的方法。
The inclusion bodies were washed with Deoxycholic acid Sodium Salt ( DOC) for several times, and then dissolved in N-Lauroyl Sarcosine Sodium ( SKL) for denaturant. After that, a good renature result was obtained by dialysis. 以脱氧胆酸钠(DOC)洗涤包涵体,然后用N&十二烷基肌氨酸钠(SKL)溶解,将溶解包涵体进行透析复性。
Development of denaturant gradient gel electrophoresis for analysis of intestinal microflora community diversity 肠道菌群多样性变性梯度凝胶电泳分析法的建立
The modified mechanism of the starch denaturant and the development of the research 淀粉改性剂的变性机理与研究现状
In recent years, much hard work has been done by scholars from all over the world. These work has mainly involved the denaturation mechanism of proteins in water, in the solution with denaturant and in the solution with other matters. 近年来,国内外学者在此方面做了大量的工作,主要涉及蛋白质在水溶液中的变性机理、在有变性剂存在下水溶液中的变性机理及在含有其它物质水溶液中的变性机理。
It was suggested that the denaturant concentration range and electrophoresis time may be the key factors for DGGE experiment. 提示合理的变性剂浓度范围和电泳时间是DGGE实验的关键因素。
The soy based wood adhesive produced by this complex denaturant can met the standard of hot water resistant type. s的初黏度指标。采用这种复合变性法制备的大豆基木材胶粘剂达到Ⅱ类(耐热水)胶的标准。
Studies on Unfolding of Bovine Serum Albumins Induced by Urea in the Presence of Denaturant by Fluorescence Phase Diagram 荧光相图法研究脲诱导牛血清白蛋白的去折叠过程
A Denaturant for Alcohol Sucrose Octaacetate 酒精变性剂蔗糖八醋酸酯
At first, RNase A was denatured by urea and dithiothreitol ( DTT). Effect of strong denaturant urea and DTT on enzyme activity and free thiol number was investigated. 首先利用脲和DTT作为变性剂,研究了脲和DTT浓度对RNaseA的酶活和自由巯基的影响规律。
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant GST-EO protein by GST affinity columns. 使用分步透析法对变性的包涵体进行复性,将复性蛋白过GST亲和层析柱得到纯化的GST-EO融合蛋白。
Based on the three-state model of protein denaturation-renaturation, an equation describing the effect of protein concentration in denaturant solution on renaturation yield of denatured protein was pre-sented. 建立在蛋白质变性-复性三态模型的基础上,给出了一个描述在变性液中变性蛋白质复性时蛋白质浓度和其复性率的关系式。
The expressed products exist mainly in the form of inclusion body and were purified by the molecular sieve and ion-exchange chromatography, then the recombinant protein was renatured by gradually decreasing the concentration of denaturant. 采用分子筛和离子交换层析对表达产物进行了纯化,获得了较高纯度的重组IL-1ra-Fcε融合蛋白,并采用逐步降低变性剂浓度的方式对融合蛋白进行了复性。
Before the purification, the protein which express in inclusion bodies required to denature ( eg guanidine hydrochloride, urea, etc.) fully dissolved under the action of the denaturant. 在包涵体表达的蛋白纯化之前需要在变性剂(如盐酸胍,尿素等)的作用下,充分溶解。
In addition, the effect of metal ion, organic solvent and denaturant on agglutinating activity were investigated. 此外,还研究了金属离子对凝集活性的影响、有机溶剂对凝集活性的影响、变性剂对凝集活性的影响。
The effect of pH value, temperature and denaturant was also investigated. 进一步研究了pH值、温度和变性剂对固定化酶活性的影响。
Candida rugosa lipases were covalently immobilized on the carriers using glutaraldehyde as a coupling agent. Various properties of the immobilized enzyme were studied such as pH, temperature and denaturant. 将制得的三代磁性纳米载体用戊二醛共价偶联固定化玫瑰假丝酵母脂肪酶。研究了固定化酶的相关性质,如pH值、温度和变性剂对固定化酶活性的影响。
The influence of reaction conditions, such as initiator concentration, monomer concentration, denaturant concentration and time on percentage graft and percentage efficiency, were investigated. 并讨论了引发剂浓度、单体浓度、变性剂浓度、时间等反应条件对接枝率(GP%)和接枝效率(GE%)的影响。