The single-stranded RNA Borna disease virus ( BDV), lacks cytopathogenicity both in vitro and in vivo. 博尔纳病病毒(BDV)是单股负链RNA病毒,在体内和体外都缺乏细胞致病性。
Methods The HeLa cell strain was cultured in a flat-bottomed 96-well plastic microtiter tray to observe cytopathogenicity ( CPE) directly. 方法采取HeLa细胞系,在96孔培养板上直接观察细胞病变;