The normal stromal cells and the PC-3 cells were cocultured using a double-layer soft agar assay. 用双层软琼脂分析法混合培养前列腺基质细胞和PC3细胞。
Objective To observe the survival and multiplication of Legionella pneumophila cocultured with Acanthamoeba polyphaga for setting up the laboratory model of such infections. 目的初步建立国内嗜肺军团菌感染原生动物自由生活阿米巴的实验感染模型。