Multipotent adipose-derived MSCs were isolated from subcutaneous, ovarian, or circumrenal adipose tissue of rabbit according to our method. Briefly, minced adipose tissue were digested with 0.1% collagenase type I and 1% BSA for 90 min at 37 ℃ with intermittent shaking. 首次采用O.1%胶原酶型I+1%BSA,消化90min的分离方法,从家兔腹股沟皮下脂肪组织,卵巢脂肪组织,或肾脏周围脂肪组织中分离得到脂肪MSCs。