Objective Type E botulism is one of main types of botulism in human beings. A polymerase chain reaction ( PCR) method was established for rapid detection of type E botulinal neurotoxin gene and identification of Clostridium botulinum type E. 目的E型肉毒中毒是人类肉毒中毒的主要型别之一,本试验旨在建立用于E型肉毒梭菌鉴定的PCR方法。