Conclusion: Single blastomere PCR is stable and reliable, and it can be used for preimplantation genetic diagnosis. 目的建立单卵裂球PCR技术,为开展单基因病的着床前遗传学诊断奠定基础。
Objective: To evaluate the influence of the blastomere survival and resumption of mitosis on the clinical outcome in frozen-thawed embryo transfer ( FET) cycles. 目的:分析冻融胚胎移植周期中胚胎卵裂球损伤程度与有丝分裂的恢复对于临床结局的影响。
Impact of blastomere number and fragment on the blastomere survival of human cryopreserved embryos 卵裂球数量及胚胎碎片对人冻胚卵裂球存活状况的影响
Establishment of a FISH Method for HBV Detection in Human Blastomere 卵裂球细胞乙型肝炎病毒FISH检测法的建立
Hence, potentially viable embryos will be discarded upon screening a single blastomere. 因此,有可能对单个卵裂球的基因筛选导致一个有活力的胚胎被丢弃。
In morula, positive LN immunoreactive substance appeared simultaneously in both blastomere and extracellular matrix. 到桑椹胚期,卵裂球内及间质中同时出现LN阳性反应物质。
The fuse rate of rabbit 32-cell stage embryo blastomere and mouse enucleated M ⅱ oocytes were 66%. 兔32-细胞期胚胎卵裂球与小鼠去核的MⅡ期卵母细胞融合率为66%。
The amplification efficiencies of multiplex nested PCR of single blastomere was ( 94.1%). 单卵裂球的扩增效率为94.1%。
Impact of blastomere survival on implantation rate of frozen-thawed embryo transfer 卵裂球的存活状况对冻融胚胎种植率的影响
Whereafter, 73 I serial and 20 II serial reconstructed eggs were gained by use of the blastomere of original cloned morula and I serial cloned morula as donor and rabbit oocyte as recipient. 随后,分别以获得的异种原代和异种继Ⅰ代克隆桑椹胚卵裂球为核供体移入去核的兔卵母细胞中,共构建继Ⅰ代重构卵73枚,继Ⅱ代重构卵20枚。
A DC pulse of 1200v/ cm and 80 μ s was applied to induce membrane fusion between a blastomere and an enucleated oocyte. 对组合卵施加一个1200伏/cm(V/cm)和80微秒(μs)的直流电脉冲,使卵裂球的细胞核导入去核卵母细胞之中。
Effect of ROS on the numbers of blastomere and mitotic index in mouse embryos 活性氧(ROS)对小鼠早期胚胎胚细胞分裂的影响
Conclusion The oviduct is the site of sperm-egg interaction and blastomere embryo development. 结论输卵管是精子与卵子结合的地方,也是胚胎早期发育的场所。
CONCLUSION: Blastomere survival status is a most important factor influencing the implantation rate of frozen-thawed embryo transfer. 结论:卵裂球的存活状况是影响冻融胚胎种植率的重要因素。
Early embryonic cell has the developmental totipotency. Every embryonic blastomere cell can develop to an intact individual separately in a proper condition. 早期胚胎卵裂球细胞具有发育全能性,每个胚胎卵裂球细胞在适宜的条件下都能单独发育为一个完整的个体。
Conclusion: LN existed in the blastomere during early mouse embryo development; 结论:早期小鼠胚胎的卵裂球中含有LN;
The average number of blastomere and score of embryo in D3 were much higher than D2, P < 0.01; D3妊娠组所移植胚胎的平均卵裂球数及评分显著高于D2组、P<0.01;
Fragile x syndrome ( 1) To detect ( CGG) n copies of FMR-1 gene of normal people in Chongqing by PCR ( 2) To detect the copies of ( CGG) n in single lymphocyte and blastomere by nested PCR. 脆性X综合征:(1)采用PCR方法检测智力正常的重庆人FMR-1基因(CGG)n拷贝数;(2)采用巢式PCR方法检测单个淋巴细胞和单个卵裂球细胞FMR-1基因(CGG)n拷贝数。
Cell embryos blastomere of Xiang pig were used as nuclear donor and Shanghai White pig used as nuclear recipient. 选用香猪2~16细胞期卵裂球作核供体,上海白猪卵母细胞作核受体。
From the difference of cleavage rate, rate of 4~ 8-cell embryo and rate of embryo of more than 8-cell, a best kind of semen for getting early embryonic blastomere cells in the lab could be screened out. 通过比较不同类型精液(鲜精、附睾精液和冷冻&解冻精液)IVF的卵裂率、4~8-细胞胚胎率以及8-细胞以上胚胎率差异,筛选出适合本实验室获得早期胚胎细胞来源的精液。
Research on the Pre-treatment Technique for Single Blastomere before Fluorescence in Situ Hybridization 单个卵裂球荧光原位杂交标本前处理的研究
Methods: Serial nuclear transfer was conducted with the goat ( Bore) ear fibroblast cells ( original group), the blastomere of goat-rabbit inter-species cloned morula ( serial I and II group) as the donor cells and rabbit oocytes as the recipient cells. 方法分别以波尔(Bore)山羊耳部成纤维细胞、山羊-兔异种克隆桑椹胚卵裂球为核供体,以兔卵母细胞为受体,进行连续核移植。
The ES cell lines establishment efficiency from a single blastomere of embryo is inversely proportional to the embryo development stages. 单卵裂球建立ES细胞的成功率与胚胎发育阶段负相关。
There was no certain regulation in the distributing of the progeny of 2-cell embryo blastomere in blastocyst. 实验结果如下:1.2-细胞胚胎两个卵裂球的后代在囊胚中分布并没有一定的规律。
Use HCL/ Tween20to fix blastomere. 采用HCL/Tween20方法进行核固定。