The results showed that: ( 1) The NRG-1 was expressed basically in normal cultured OPCs; 结果显示:(1)正常培养的OPCs中有基础性NRG-1的表达;
Two or more binary sequences are called as a set of odd-periodic complementary binary sequences ( OPCS) if the sum of their respective odd-periodic autocorrelation function is a delta function. 如果两个以上二元序列的奇周期自相关函数之和是一个冲击函数,这些序列称为奇周期二元互补序列集(简记为OPCS)。
The OPCs extensively grew and proliferated in the primary culture, showing the typical appearance of precursor cells. Under the observation of microscope, the OPCs were seen with round soma and bipolar processes of their bodies. 结果1.原代培养中OPCs大量增殖生长呈典型的前体细胞形态,镜下见其胞体呈近圆形,为折光性强的小亮点,胞体具有双极细胞突起。
Until the present time, no one has investigated whether intracellular calcium signals were involved in OPC-induced apoptosis at the early stage of apoptotic process, and whether the ER-associated pathway participated in apoptosis induced by OPCs. 但是尚未有实验研究内质网通路是否参与了OPCs诱导的凋亡,以及钙信号是否在OPCs诱导的凋亡早期的信号传递中发挥作用。
Because of their wide uses, OPCs are also widespread environmental pollutants and potent toxicants to many kinds of organisms, including human beings. 由于其广泛的应用,OPCs也是分布广泛的环境污染物,对包括人类在内的多种生物具有很强的毒性。
The transplantation of OPCs is able to benefit the preservation of white matter, to ameliorate the axonal myelination of injured spinal cord, to improve the survival of neurons and axons and reduce the glial reaction as well. OPCs移植治疗大鼠脊髓损伤有利于损伤脊髓组织内白质存留,改善损伤脊髓轴突髓鞘化,促进损伤脊髓组织内神经元和轴突存活及减轻胶质反应。
The isolated OPCs are found to be able to survive and continue to grow in vitro. These OPCs still retain the proliferative ability and further differentiate and maturate to oligodendrocytes. 3. 研究结果表明分离后OPCs能够在体外条件下继续存活生长,保持着良好的分裂增殖能力,并且能够在体外定向分化为成熟的少突胶质细胞。
Around 9-10 days in vitro, the distinct stratification of glial cells formed in the primary culture. The upper layer consisted of clustered-growing OPCs and the bottom layer was mostly constituted of confluent astrocytes. 原代培养第9-10天时形成明显的细胞分层,上层为成簇生长的OPCs,下层为融合成片的星形胶质细胞。
As one of the indispensable premises for myelin, OPCs migration has gradually been widely concerned. 作为髓鞘形成的必须前提,OPCs迁移过程得到了广泛的关注。
In the system of cell culture, OPCs have proved to have the potency of cell differentiation, such as differentiating into oligodendrocyte cells, astrocytes, even neurons. 在培养细胞系统,已经发现OPC有分化成多种细胞的潜能,它能够分化成少突胶质细胞和星形胶质细胞[6],甚至还能分化成神经元[7]。
After the primary culture in vitro, the OPCs were further dissociated by the shaking process and differential adhesion. Then, the separated OPCs were also identified and analyzed by the immunocytochemical technique. 3. 实验采用振荡分离和差速贴壁等方法进一步分离OPCs,通过免疫细胞化学技术对分离后的OPCs进行分析鉴定。
Since NOV was the important regulating factor of nerve system development and regeneration, and IGF was the encode binding protein, we want to see if the NOV have the effects on promoting the regeneration and function recovery of OPCs after OGD. 既然NOV基因是动物神经系统发育、再生的重要调控因子,IGF系其编码结合蛋白,将其应用于OPCs的研究,可以观察其对OPCs发育分化及缺血、缺氧性白质损伤的作用。
Indeed, OPCs express many neurotransmitter receptors which are invovled in the regulation of cellular migration. Glutamate, as an important neurotransmitter, is widely distributed in whole brain and regulates numerous neurophysiological activities. 事实上,OPCs表达多种神经递质受体并可以介导OPCs的迁移。谷氨酸作为脑内广泛分布的一类神经递质,参与调节了众多神经生理功能。
In recent years, more and more studies have indicated that induction of apoptosis is a new toxic effect of OPCs, in vitro or in vivo, and that apoptotic cell death may play an important role in OPC-mediated impairing effects. 近年来,越来越多的体外和在体的研究结果表明诱导凋亡是OPCs的一种新发现的毒理学机制,并且凋亡性的细胞死亡在OPCs诱导的损伤效应中起重要作用。
The OPCs culture is able to be well established in vitro. Timely primary culture, suitable shaking process and differential adhesion together do efficiently separate the OPCs in the primary culture. 2. 结论1.本实验建立了OPCs体外培养体系,通过适时的原代培养、恰当的振荡分离及差速贴壁等方法处理能够有效地分离获得OPCs。
The number and immune reaction of OPCs increased after nerve degenerative disease. 在神经退行性疾病如侧束硬化症时NG2+细胞数量增多而且免疫反应显著加强。
Organophosphorus compounds ( OPCs) are frequently utilized in agriculture, industry and medicine all over the world. 有机磷化合物(organophosphoruscompounds,OPCs)在全世界范围内被广泛应用于工业、农业和医学领域中。
Therefore, until the present time, toxic effects and toxicological mechanisms of OPCs have been attended to and been studied in vitro or in vivo by many researchers. 因此,迄今为止,OPCs的毒性效应和毒理学机制一直是众多科学研究者关注的对象,并进行了许多这方面的体外和在体研究。
The recovery of hindlimb locomotor activity of the injured rats in the OPCs transplant group was much better than that of the transplant control group and plain injury group. OPCs移植治疗组大鼠后肢运动功能的恢复情况明显好于移植对照组和单纯损伤组。
The findings of transmission electron microscopy further demonstrated the improved axonal myelination in the lesioned spinal cord of the OPCs transplant group. 透射电镜观察结果显示OPCs移植治疗组大鼠损伤脊髓组织内轴突髓鞘化有明显的改善。
The transplantation of OPCs enhances the recovery of hindlimb locomotor activity and conductive ability of the lesioned spinal cord after SCI. 4. OPCs移植治疗能够促进脊髓损伤后大鼠后肢运动功能和脊髓神经传导功能的恢复。
As immature cells, OPCs have more advantages over mature oligodendrocytes to be a transplant, and OPCs are also less influenced by the hostile microenviroment of the lesion areas as compared with stem cells. OPCs作为移植细胞比成熟少突胶质细胞更具有优势,也不像干细胞那样容易受到移植微环境的不利影响。
The outcome of electrophysiological experiments indicated that the recovery of the conductive ability of injured spinal cord in the OPCs transplant group was also better than that of the transplant control group and plain injury group. 神经电生理检测结果表明OPCs移植治疗组大鼠损伤脊髓神经传导功能的恢复亦明显好于移植对照组和单纯损伤组。
Oligodendrocyte precursor cells ( OPCs) are immature oligodendrocytes which are able to proliferate, migrate, and finally differentiate into mature oligodendrocytes and produce myelin. 少突胶质前体细胞(OligodendrocytePrecursorCells,OPCs)是未成熟的少突胶质细胞,能够在体内增殖、迁移、最后分化为成熟少突胶质细胞并形成髓鞘发挥功能。