The using of co-transformation system, site-specific recombination system ( FLP/ FRT? Cre/ lox? R/ RS and Gin/ gix systems) and transposable element system ( Ac/ Ds transposable element system) in breeding marker-free transgenic plants were reviewed in this paper. 本文综述了共转化系统、位点特异性重组系统(包括FLP/FRT、Cre/lox、R/RS及Gin/gix系统)和转座子系统(Ac/Ds转座子系统)在培育无选择标记转基因植物中的应用。