Purification of CALB by Ammonium Sulphate-Actone Concerted Precipitation Method 硫酸铵-丙酮协同沉淀法纯化南极假丝酵母产脂肪酶
The work consists of three parts: ( 1) Investigating the conformation changes of Candida antarctica lipase B ( CALB) in several polar and non-polar organic solvents by molecular dynamics simulation. 研究分为三部分:(1)通过分子动力学模拟方法研究南极甲丝酵母脂肪酶B在极性有机溶剂和非极性有机溶剂中的构象活性变化规律。
For its broad spectrum of substrate acceptance and enantioselectivity, Candida antarctica lipase B ( CALB) has been widely used in the fields of food, chemicals, drug precursors synthesis and bio-energy. 南极假丝酵母脂肪酶B(CandidaAntarcticalipaseB,CALB)具有广谱底物接受性和对映体选择性,广泛应用于食品、化工、前体药物的合成和生物能源等领域。
The enzymatic characteristics of the recombinant CALB were also determined. 对重组CALB进行了初步的酶学性质分析。
It has a strong catalytic activity in non-water-soluble and water-soluble systems. CALB shows greater superiority than other lipases in a variety of reactions because of its stability and specificity. 它对于非水溶性和水溶性物质都有很强的催化活力,并且具有良好的稳定性,在转酯等反应中显示出优于其他脂肪酶的性质。
In the enzyme property assay of CALB displayed on Pichia yeast. 对毕赤酵母表面展示CALB全细胞催化剂的酶学特性进行研究。